| 產品編號 | bsm-60630R |
| 英文名稱 | WWOX Recombinant Rabbit mAb |
| 中文名稱 | |
| 別 名 | D16S432E; FOR; FOR II protein; FRA16D; Fragile 16D oxido reductase; fragile site FRA16D oxidoreductase; HHCMA 56; HHCMA56; PRO0128; putative oxidoreductase; WOX 1; WOX1; WW domain containing oxidoreductase; WW domain-containing protein WWOX; WWOX2; WWOX4; WWOX8; WWOX. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | F11B6 |
| 交叉反應 | Human,Rat (predicted: Mouse) |
| 產品應用 | WB=1:500-1:2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1:25,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 | 作為腫瘤抑制因子，在細胞凋亡中發(fā)揮作用?？赡芘cp53/TP53協同作用以控制基因毒性應激誘導的細胞死亡。在TGFB1信號傳導和TGFB1介導的細胞死亡中發(fā)揮作用。也可能在腫瘤壞死因子（TNF）介導的細胞死亡中發(fā)揮作用。抑制Wnt信號，可能是通過在細胞質中隔離DVL2。 |
| 產品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: MCF-7, 2: Mouse ovary, 3: Rat brain
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 47 kDa
Observed MW: 47 kDa
Tissue: Human cerebrum
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 4°C overnight
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60630R
Tissue: Human testis
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60630R
Tissue: Rat cerebrum
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60630R
Cell line: A549
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for bsm-60630R
Cell line: MCF-7
Fixation: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:50
Secondary Ab: Goat Anti-Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60630R
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
