| 產品編號 | bs-23809R |
| 英文名稱 | MyoD1/Myf3 Rabbit pAb |
| 中文名稱 | 肌原調節(jié)蛋白抗體 |
| 別 名 | Myogenic differentiation 1; AI503393; bHLHc1; MD1; MGC156574; MYF3; MYOD; MYOD1; PUM; MYOD1_HUMAN; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3. |
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Specific References (2) | bs-23809R has been referenced in 2 publications.
[IF=8.713] Zhao-Bo Luo. et al. Fecal transplant from myostatin deletion pigs positively impacts the gut-muscle axis. ELIFE. 2023; 12: e81858 WB ; Mouse.
[IF=8.025] Siqi Liu. et al. Estrogen-mediated oar-miR-485-5p targets PPP1R13B to regulate myoblast proliferation in sheep. INT J BIOL MACROMOL. 2023 May;236:123987 WB ; Ewe.
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| 研究領域 | 細胞生物 發(fā)育生物學 轉錄調節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MyoD1/Myf3: 1-100/320 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008] Function: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins. Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1 and CDK9. Interacts with DDX5. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation. Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function. Ubiquitinated on the N-terminus; which is required for proteasomal degradation. Similarity: Contains 1 bHLH (basic helix-loop-helix) domain. SWISS: P15172 Gene ID: 4654 Database links: Entrez Gene: 374048 Chicken Entrez Gene: 4654 Human Entrez Gene: 17927 Mouse Entrez Gene: 443405 Sheep Omim: 159970 Human SwissProt: P16075 Chicken SwissProt: P15172 Human SwissProt: P10085 Mouse SwissProt: P29331 Sheep Unigene: 181768 Human Unigene: 1526 Mouse Unigene: 9493 Rat |
| 產品圖片 |
Sample: Lane 1: Skeletal muscle (Mouse) Lysate at 40 ug Lane 2: Skeletal muscle (Rat) Lysate at 40 ug Primary: Anti-MyoD1/Myf3 (bs-23809R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 40 kD
Sample: Lane 1: Liver (Mouse) Lysate at 40 ug Lane 2: Heart (Mouse) Lysate at 40 ug Primary: Anti-MyoD1’Myf3 (bs-23809R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 40 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
