| 產(chǎn)品編號 | bs-18196R |
| 英文名稱 | LCN8 Rabbit pAb |
| 中文名稱 | 附睪特異蛋白8抗體 |
| 別 名 | Epididymal specific lipocalin 8; Epididymal-specific lipocalin-8; LCN5; Lcn8; LCN8_HUMAN. |
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Specific References (1) | bs-18196R has been referenced in 1 publications.
[IF=3.885] Ziyang Sheng. et al. Zika virus disrupts the barrier structure and Absorption/Secretion functions of the epididymis in mice. Plos Neglect Trop D. 2021 Mar;15(3):e0009211 IHC ; Mouse.
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| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 發(fā)育生物學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 17 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LCN8: 51-150/175 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Members of the lipocalin family, such as LCN8, have a common structure consisting of an 8-stranded antiparallel beta-barrel that forms a cup-shaped ligand-binding pocket or calyx. Lipocalins generally bind small hydrophobic ligands and transport them to specific cells (Suzuki et al., 2004 [PubMed 15363845]).[supplied by OMIM, Aug 2009] Function: May play a role in male fertility. May act as a retinoid carrier protein within the epididymis. Subcellular Location: Secreted. Similarity: Belongs to the calycin superfamily. Lipocalin family. SWISS: Q6JVE9 Gene ID: 138307 Database links: Entrez Gene: 138307 Human Omim: 612902 Human SwissProt: Q6JVE9 Human Unigene: 323991 Human |
| 產(chǎn)品圖片 |
Sample:
Epididymis (Mouse) Lysate at 40 ug
Primary: Anti-LCN8 (bs-18196R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 27 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LCN8) Polyclonal Antibody, Unconjugated (bs-18196R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
