| 產(chǎn)品編號 | bs-13609R |
| 英文名稱 | DAZ4 Rabbit pAb |
| 中文名稱 | 無精癥缺失基因4抗體 |
| 別 名 | DAZ4; Deleted in azoospermia 4; Deleted in azoospermia protein 4; DAZ4_HUMAN. |
| 研究領域 | 細胞生物 發(fā)育生物學 干細胞 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DAZ4: 51-150/579 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Spermatogenesis is the process by which male spermatogonia develop into mature spermatozoa. DAZ (deleted in azoospermia) are RNA-binding proteins that play an essential role in spermatogenesis. DAZ proteins influence the first stages of spermatogenesis and the maintenance of germ cell populations. DAZ proteins (DAZ1, DAZ2, DAZ3, DAZ4 and DAZ5) are encoded by separate genes on chromosome Y, each of which contain an AZFc domain in their coding region. DAZ proteins localize to the nucleus of spermatogonia, but relocate to the cytoplasm during meiosis. DAZ proteins contain an RRM (RNA recognition motif) domain that may regulate mRNA translation by binding to the 3’UTR. Deletions in the genes encoding DAZ proteins may cause azoospermia or oligospermia which can lead to male infertility. DAZ4 (deleted in azoospermia 4), also known as pDP1680 or pDP1681, is a 579 amino acid testis specific protein that contains nine DAZ-like domains and two RNA recognition motifs (RRM). DAZ4 exists as two alternatively spliced isoforms. Function: Deleted in azoospermia 4 is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that plays an essential role in spermatogenesis. It may act by binding to the 3'-UTR of mRNAs and regulating their translation. Subcellular Location: Cytoplasm. Nucleus. Similarity: Belongs to the RRM DAZ family. Contains 9 DAZ-like domains. Contains 2 RRM (RNA recognition motif) domains. SWISS: Q86SG3 Gene ID: 57135 Database links: Entrez Gene: 57135 Human Omim: 400003 Human SwissProt: Q86SG3 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Rat Testis tissue lysates
Lane 2: Rat Stomach tissue lysates
Lane 3: Human HepG2 cell lysates
Lane 4: Human A549 cell lysates
Primary: Anti-DAZ4 (bs-13609R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 62 kDa
Sample:
Lymph node (Mouse) Lysate at 40 ug
Testis (Mouse) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Epididymis (Mouse) Lysate at 40 ug
Primary: Anti- DAZ4 (bs-13609R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
