| 產(chǎn)品編號 | bs-4249R |
| 英文名稱 | P2X3 Rabbit pAb |
| 中文名稱 | 三磷酸腺苷受體受體P2X3抗體 |
| 別 名 | ATP receptor; ATP receptor; MGC129956; P2RX3; P2RX3_HUMAN; P2X purinoceptor 3; P2X receptor subunit 3; P2X3; Purinergic receptor; Purinergic receptor P2X ligand gated ion channel 3; Purinergic receptor P2X3; Purinoceptor P2X3. |
| 研究領域 | 腫瘤 免疫學 神經(jīng)生物學 通道蛋白 細胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Pig,Horse) |
| 產(chǎn)品應用 | Flow-Cyt=1ug/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 44 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human P2X3: 241-340/397 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
P2RX3 is a member of the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and transduces ATP-evoked nociceptor activation. P2RX3 is important for peripheral pain responses, and controls urinary bladder volume reflexes. Function: Receptor for ATP that acts as a ligand-gated ion channel. Subunit: Homo- or heteropolymers. Subcellular Location: Membrane; Multi-pass membrane protein. Similarity: Belongs to the P2X receptor family. SWISS: P56373 Gene ID: 5024 Database links: Entrez Gene: 5024 Human Entrez Gene: 228139 Mouse Omim: 600843 Human SwissProt: P56373 Human SwissProt: Q3UR32 Mouse Unigene: 146738 Human Unigene: 440375 Mouse Unigene: 448935 Mouse Unigene: 10388 Rat |
| 產(chǎn)品圖片 |
Blank control: U87MG.
Primary Antibody (green line): Rabbit Anti-P2X3 antibody (bs-4249R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
